RP: Microscopy

What is the first step in setting up a microscope for viewing a specimen?

• Turn off the light source.

• Place the specimen on the stage.

• Change the objective lens to the highest magnification.

• Adjust the mirror to maximize light.

Why is iodine used when preparing an onion skin slide?

• To enhance the visibility of cell walls.

• To clean the slide surface.

• To prevent the slide from drying out.

• To stain the starch in the cells, making them more visible.

When preparing a slide with onion skin, what is crucial to avoid?

• Using too much iodine.

• Allowing air bubbles under the coverslip.

• All of the above.

• Placing the coverslip upside down.

What should you do if the microscope's image is not in focus?

• Switch to the highest magnification lens.

• Adjust the fine focus dial for a clearer image.

• Turn off the microscope and restart the setup.

• Remove and reset the specimen on the stage.

How is the stage adjusted to begin focusing under the microscope?

• It is raised to the highest position.

• It is removed and cleaned before focusing.

• It is moved to the lowest position.

• It is not adjusted; the lens is moved instead.

What is the purpose of the coarse focus dial on a microscope?

• To move the stage sideways.

• To adjust the light intensity.

• To switch between different lenses.

• To make large adjustments in focus.

How do you ensure that the lens does not touch the slide when increasing magnification?

• Lower the stage as much as possible.

• Raise the lens and observe from the side.

• Apply more light using the light source.

• Clean the lens with a soft cloth.

What should you do after increasing the microscope's magnification?

• Remove the specimen and reapply iodine.

• Refocus using the fine focus dial.

• Turn off the microscope's light source.

• Switch back to the lowest magnification.

What unit of measurement is commonly used in microscopy to measure cell size?

• Inches

• Micrometers

• Centimeters

• Millimeters

How do you calculate the size of a cell when you know the field of view?

• Multiply the number of cells by the field of view.

• Add the size of all visible cells and divide by the field of view.

• None of the above.

• Divide the field of view by the number of cells across it.