Insulin from E.Coli

Insulin from E.Coli

Assessment

Interactive Video

Science

11th Grade

Practice Problem

Hard

NGSS
HS-LS1-1, HS-PS1-2

Standards-aligned

Created by

Dina Kruger

FREE Resource

Standards-aligned

NGSS.HS-LS1-1
,
NGSS.HS-PS1-2

5 questions

Show all answers

1.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What was the primary source of insulin before recombinant DNA technology?

Plant extracts

Laboratory-grown bacteria

Synthetic chemical compounds

Cow and pig pancreas

2.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the first step in the recombinant DNA technology for producing insulin?

Purification of insulin

Insertion of the insulin gene into a host

Expression of the insulin gene

Identification and isolation of the insulin gene

Tags

NGSS.HS-LS1-1

3.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Which enzyme is used to seal the cut ends of DNA during vector construction?

Helicase

Ligase

Nuclease

Polymerase

4.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the purpose of adding lactose in the insulin production process?

To purify the insulin

To induce the expression of the insulin gene

To act as a nutrient for bacteria

To stabilize the insulin protein

5.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

How are the insulin A and B chains finally activated?

By forming disulfide bonds under certain conditions

By adding more beta-galactosidase

By mixing in a specific nutrient medium

By heating to a specific temperature

Tags

NGSS.HS-PS1-2

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