Insulin from E.Coli

What was the primary source of insulin before recombinant DNA technology?

• Plant extracts

• Laboratory-grown bacteria

• Synthetic chemical compounds

• Cow and pig pancreas

What is the first step in the recombinant DNA technology for producing insulin?

• Purification of insulin

• Insertion of the insulin gene into a host

• Expression of the insulin gene

• Identification and isolation of the insulin gene

Tags

NGSS.HS-LS1-1

Which enzyme is used to seal the cut ends of DNA during vector construction?

• Helicase

• Ligase

• Nuclease

• Polymerase

What is the purpose of adding lactose in the insulin production process?

• To purify the insulin

• To induce the expression of the insulin gene

• To act as a nutrient for bacteria

• To stabilize the insulin protein

How are the insulin A and B chains finally activated?

• By forming disulfide bonds under certain conditions

• By adding more beta-galactosidase

• By mixing in a specific nutrient medium

• By heating to a specific temperature

Tags

NGSS.HS-PS1-2