Understanding DNA Replication

Where does DNA replication occur in eukaryotic cells?

• Cytoplasm

• Mitochondria

• Nucleus

• Ribosome

Which enzyme is responsible for unzipping the DNA strands?

• Primase

• Ligase

• DNA Polymerase

• Helicase

What is the role of primase in DNA replication?

• Creating RNA primers

• Gluing DNA fragments

• Proofreading the DNA

• Building new DNA strands

Which direction does DNA polymerase build new DNA strands?

• Random direction

• 3' to 5'

• 5' to 3'

• Both directions

Which enzyme prevents DNA strands from re-annealing after being unzipped?

• DNA Polymerase

• Primase

• SSB Proteins

• Topoisomerase

What are Okazaki fragments?

• DNA fragments on the leading strand

• Enzymes in DNA replication

• RNA primers

• DNA fragments on the lagging strand

What is the function of ligase in DNA replication?

• Creating RNA primers

• Proofreading DNA

• Sealing gaps between DNA fragments

• Unzipping DNA strands

What is the significance of the 5' to 3' direction in DNA replication?

• It is the only direction DNA polymerase can build new strands

• It is the direction RNA primers are placed

• It is necessary for the unzipping of DNA

• It is the direction ligase works

Why is DNA replication called semi-conservative?

• Each new DNA molecule contains one old and one new strand

• Only one strand is used as a template

• It conserves all original DNA molecules

• Each strand is partially conserved

What does the proofreading ability of DNA polymerase prevent?

• Mismatch of DNA bases

• Formation of RNA primers

• Supercoiling of DNA

• Unzipping of DNA