POST TEST ELEKTROFORESIS P2

What is electrophoresis used for?

•  DNA synthesis

• Protein denaturation

• Separation of molecules

• Cell division

Answer explanation

Electrophoresis is used for the separation of molecules, allowing them to move through a gel based on their size and charge.

1. Which of the following is a common medium used in gel electrophoresis?

• Agarose

• Sand

• Plastic

• Rubber

Answer explanation

Agarose is a common medium used in gel electrophoresis, making it the correct choice among the options provided.

What is the purpose of the buffer solution in electrophoresis?

• To keep the gel moist

• To conduct electricity

• To stain the samples

• To denature proteins

Answer explanation

The purpose of the buffer solution in electrophoresis is to conduct electricity.

During electrophoresis, DNA molecules migrations from

• negative to positive

• positive to negative

•  top to bottom

• bottom to top

Answer explanation

During electrophoresis, DNA molecules migrate from negative to positive electrodes due to the negatively charged phosphate backbone of DNA being attracted to the positive electrode.

What is used to visualize DNA after electrophoresis?

• X-ray film

• UV light

• Regular light

•  Infrared light

Answer explanation

UV light is used to visualize DNA after electrophoresis.

1. Which of these molecules is commonly separated using electrophoresis?

• Glucose

• Water

• DNA

•  Salt

Answer explanation

DNA is commonly separated using electrophoresis due to its negatively charged phosphate backbone, allowing it to move towards the positive electrode.

1. What determines the rate of movement of molecules in gel electrophoresis?

• Molecular weight

• • Color of the sample

• Volume of the sample

•  Temperature of the room

Answer explanation

The rate of movement of molecules in gel electrophoresis is determined by their molecular weight.

1. What is the charge of DNA molecules that allows them to move in an electric field?

• Positive

• Negative

• Neutral

• • Variable

Answer explanation

DNA molecules are negatively charged due to the phosphate groups in their structure, allowing them to move towards the positive electrode in an electric field.

What is the main component of agarose gel?

• • Proteins

• Polysaccharides

• Lipids

• • Nucleic acids

Answer explanation

The main component of agarose gel is polysaccharides.

What is the purpose of adding a loading dye to the samples?

• To increase the viscosity

• To color the gel

• •  To track the progress of the samples

•  To denature the samples

Answer explanation

To color the gel