What does PCR stand for?

• Protein Cell Reaction

• Polymerase Cell Reaction

• Protein Chain Reaction

• Polymerase Chain Reaction

Which department does Osama Mohammed work in?

• Ministry of Health

• Ministry of Science & Technology

• Ministry of Education

• Ministry of Agriculture

What is the purpose of PCR?

• To amplify a specific protein region

• To amplify a specific DNA region

• To amplify a specific lipid region

• To amplify a specific RNA region

What is included in the reaction mixture of PCR?

• Lipids

• ATP

• Two target specific primers

• RNA polymerase

How many major steps are there in a PCR?

• Three

• One

• Four

• Two

What is the first step in a PCR cycle?

• Template denaturation

• DNA replication

• Primer extension

• Primer annealing

What is the purpose of annealing in PCR?

• To amplify DNA

• To copy DNA using primers

• To heat the test tube

• To elongate primer molecules

How is the annealing temperature calculated?

• Tanneal = Tm_primer / 4°C

• Tanneal = Tm_primer + 4°C

• Tanneal = Tm_primer - 4°C

• Tanneal = Tm_primer x 4°C

What happens during the extension step in PCR?

• Heating the test tube

• Copying DNA using primers

• Amplifying DNA

• Elongating primer molecules

In which stage of PCR is the amount of product doubled at every cycle?

• Plateau

• Levelling off stage

• Exponential amplification

• None of the above