Genetic Engineering Quiz

Explain the role of the guide RNA (gRNA) in the CRISPR-Cas9 mechanism.

• It binds to the target DNA sequence and guides the Cas9 enzyme to the correct location.

• It cuts the DNA at the target site.

• It repairs the DNA after it has been cut.

• It deactivates the Cas9 enzyme.

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DOK Level 3: Strategic Thinking

Describe a strategic plan for using CRISPR-Cas9 to correct a genetic mutation in a human cell. Include the steps and reasoning behind each step.

• Identify the mutation, design a gRNA, introduce the CRISPR-Cas9 system into the cell, and verify the correction.

• Identify the mutation, design a gRNA, introduce the CRISPR-Cas9 system into the cell, and observe the cell for changes.

• Identify the mutation, design a gRNA, introduce the CRISPR-Cas9 system into the cell, and wait for natural repair mechanisms.

• Identify the mutation, design a gRNA, introduce the CRISPR-Cas9 system into the cell, and use antibiotics to select for corrected cells.

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DOK Level 4: Extended Thinking

Evaluate the potential risks and benefits of using genetic engineering in agriculture.

• Benefits include increased yield and pest resistance; risks include potential environmental impact and loss of biodiversity.

• Benefits include increased yield and pest resistance; risks include higher costs and reduced crop quality.

• Benefits include reduced pesticide use and improved nutritional content; risks include potential health effects and ethical concerns.

• Benefits include reduced pesticide use and improved nutritional content; risks include higher costs and reduced crop quality.

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DOK Level 3: Strategic Thinking

Analyze the ethical implications of using CRISPR-Cas9 in gene therapy for human embryos.

• It could lead to designer babies and exacerbate social inequalities.

• It could eliminate genetic diseases and improve quality of life.

• It could lead to unforeseen genetic complications and ethical dilemmas.

• All of the above.

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DOK Level 4: Extended Thinking

Evaluate the potential future implications of genetic engineering on biodiversity.

• It could lead to a reduction in genetic diversity and the dominance of engineered species.

• It could enhance biodiversity by introducing new traits into wild populations.

• It could have no significant impact on biodiversity.

• It could lead to the extinction of non-engineered species.

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DOK Level 3: Strategic Thinking

Critically assess the role of CRISPR-Cas9 in advancing personalized medicine.

• It allows for precise genetic modifications tailored to individual patients, potentially leading to more effective treatments.

• It allows for broad genetic modifications that can be applied to large populations, potentially leading to more effective treatments.

• It allows for precise genetic modifications tailored to individual patients, potentially leading to more side effects.

• It allows for broad genetic modifications that can be applied to large populations, potentially leading to more side effects.

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DOK Level 3: Strategic Thinking

Evaluate the potential societal impacts of genetic engineering technologies becoming widely accessible.

• It could democratize access to advanced medical treatments but may also raise ethical and privacy concerns.

• It could democratize access to advanced medical treatments but may also lead to increased healthcare costs.

• It could limit access to advanced medical treatments and raise ethical and privacy concerns.

• It could limit access to advanced medical treatments and lead to increased healthcare costs.

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DOK Level 3: Strategic Thinking

CRISPR-cas9 is a genetic modification technique that edits parts of the genome of an organism. Using this technique scientists can add, remove, or modify sections of the DNA sequence.

How can scientists use this technique to control gene expression?

• by activating or deactivating specific genes

• by calculating the number of genes different organisms produce

• by identifying how closely related one individual is to another

• by determining the number of chromosomes in an organism