BIOL 1208 Final Exam Review

Assume the DNA in the final lane is linear. How many cuts were needed to produce the number of fragments seen?

Which instrument is used to determine how much light is absorbed by a solution?

• A spectrophotogram

• A spectrogram

• A spectroscope

• A spectrometer

• None of the above

Tags

NGSS.HS-PS4-5

Select all of the following which were NOT used as independent variables in the Enzymes Lab Series.

• Catechol concentration

• Temperature

• Catecholase concentration

• Sugar concentration

• None of the above

Suppose a solution of Spirulina has a concentration of 875 mM. You are given a standard curve whose equation is y = mx + b, where the slope of the graph is 10 and the y-intercept is 15. What is the absorbance (in ODU) of the Spirulina solution?

Answer explanation

y = mx + b

Absorbance = [(slope)(concentration)] + y-intercept

Absorbance = [(10)(875)] + 15

Absorbance = [8750] + 15

Absorbance = 8765 ODU

Which pipette with a calibration mark is designed to deliver a single precise volume?

Suppose there are 4 tubes of cobalt chloride A, B, C, and D with the following absorbances (in ODU) at 200 nm: 10, 5, 37, 22. Which tube of cobalt chloride is the most concentrated?

• A

• B

• C

• D

• Too little information to determine

Assume the DNA in the second lane is circular. How many cuts were needed to produce the number of fragments seen?

On a standard curve, which of the following is DIRECTLY related to concentration?

• Transmittance

• Molarity

• Wavelength

• Absorbance

• Fluorescence

Which type of pipette is designed to transfer drops or non-specific volumes?

When you spread E. coli onto TSA plates, which treatment served as the control for the experiment?

• Tet ONLY

• Tet + amp

• Amp ONLY

• UV + tet

• UV + amp