11 ATAR DNA extraction-validation revision

What is used to break apart the cells from each other when extracting dna from fruit?

• Lemon juice

• Vinegar

• Salt water

• Sugar water

What is added to the fruit soup to rupture the cell membranes and release the dna?

• Vinegar

• Bleach

• Ammonia

• Detergent

What is used to make the dna insoluble during the extraction process?

• Hot water

• Cold water

• Methanol

• Ethanol

What is the purpose of adding salt to the fruit soup during dna extraction?

• To remove the dna

• To make the dna more soluble

• To clump the dna together

• To break down the dna

What is the role of protease enzyme in the dna extraction process?

• To remove the dna

• To increase the dna length

• To break down the proteins around the dna

• To make the dna more soluble

What is the purpose of adding ice cold ethanol to the mixture during dna extraction?

• To break down the dna

• To slow down the action of enzymes

• To speed up the action of enzymes

• To remove the dna

Why do you not crush the strawberries too much?

• To maintain the natural colour of the strawberries

• It will cause the DNA to degrade

• To avoid releasing excess sugar

• To prevent contamination if you break the bad

What does crushing the strawberries do?

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It releases the nuclei where the DNA is stored.

What product in the process, breaks down the fatty membranes of the cell?

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The soap, found in the detergent

When the nuclear membrane is broken down, what is released into the solution?

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DNA