Biological Skills DNA & Extraction Methods
University
•67 Qs
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Biological Skills DNA & Extraction Methods
Quiz
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Biology
•
University
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Practice Problem
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Medium
FARNIDAH JASNIE
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67 questions
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1.
MULTIPLE CHOICE QUESTION
20 sec • 1 pt
What is the purpose of a buffer system in electrophoresis?
Alternate the current
Carry the current, maintain a stable pH for the separation of molecules and protect the sample
disrupt the contaminants
stop the migration process
2.
MULTIPLE CHOICE QUESTION
20 sec • 1 pt
How can DNA fragments be separated using gel electrophoresis ?
DNA carries a negative charge and large fragments are pulled more strongly than small fragments towards the positive electrode
DNA carries a negative charge and small fragments are able to travel more quickly than large towards the positive electrode
DNA carries a positive charge and large fragments are pulled more strongly than small fragments towards the negative electrode
DNA carries a positive charge and small fragments are able to travel more quickly than large towards the negative electrode
3.
MULTIPLE CHOICE QUESTION
20 sec • 1 pt
Where would you find DNA, the genetic material, in an animal cell?
In the centriole
Only in the mitochondria
In the mitochondria and the nucleus
In the cytoplasm
4.
MULTIPLE CHOICE QUESTION
20 sec • 1 pt
What is the first step in isolating DNA?
Generating genomic DNA fragments with restriction endonucleases
Introducing recombinant DNA into an organism by any means
Overexpressing proteins in E. coli
Lysing the cells in the sample
5.
MULTIPLE CHOICE QUESTION
20 sec • 1 pt
What is the role of ethanol in DNA extraction?
To break down cell walls
To precipitate DNA from the solution
To neutralize the pH
To increase the solubility of the DNA
6.
MULTIPLE CHOICE QUESTION
20 sec • 1 pt
Which of the following statements accurately describes the role of centrifugation in DNA extraction?
It lyses the cells to release DNA.
It selectively precipitates DNA from the solution.
It separates DNA fragments based on size.
It separates heavier cellular components from the DNA suspension.
7.
MULTIPLE CHOICE QUESTION
30 sec • 1 pt
Why do we use salt for this lab?
to dissolve the lipids in the nuclear envelope
We don't use salt.
removes protein that is chemically joined to DNA
DNA will precipitate (form) in less dense ethanol layer
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