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Biological Skills DNA & Extraction Methods

Authored by FARNIDAH JASNIE

Biology

University

Used 4+ times

Biological Skills DNA & Extraction Methods
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67 questions

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1.

MULTIPLE CHOICE QUESTION

20 sec • 1 pt

What is the purpose of a buffer system in electrophoresis?

Alternate the current

Carry the current, maintain a stable pH for the separation of molecules and protect the sample

disrupt the contaminants

stop the migration process

2.

MULTIPLE CHOICE QUESTION

20 sec • 1 pt

How can DNA fragments be separated using gel electrophoresis ?

DNA carries a negative charge and large fragments are pulled more strongly than small fragments towards the positive electrode

DNA carries a negative charge and small fragments are able to travel more quickly than large towards the positive electrode

DNA carries a positive charge and large fragments are pulled more strongly than small fragments towards the negative electrode

DNA carries a positive charge and small fragments are able to travel more quickly than large towards the negative electrode

3.

MULTIPLE CHOICE QUESTION

20 sec • 1 pt

Where would you find DNA, the genetic material, in an animal cell?

In the centriole

Only in the mitochondria

In the mitochondria and the nucleus

In the cytoplasm

4.

MULTIPLE CHOICE QUESTION

20 sec • 1 pt

What is the first step in isolating DNA?

Generating genomic DNA fragments with restriction endonucleases

Introducing recombinant DNA into an organism by any means

Overexpressing proteins in E. coli

Lysing the cells in the sample

5.

MULTIPLE CHOICE QUESTION

20 sec • 1 pt

What is the role of ethanol in DNA extraction?

To break down cell walls

To precipitate DNA from the solution

To neutralize the pH

To increase the solubility of the DNA

6.

MULTIPLE CHOICE QUESTION

20 sec • 1 pt

Which of the following statements accurately describes the role of centrifugation in DNA extraction?

It lyses the cells to release DNA.

It selectively precipitates DNA from the solution.

It separates DNA fragments based on size.

It separates heavier cellular components from the DNA suspension.

7.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Why do we use salt for this lab?

to dissolve the lipids in the nuclear envelope

We don't use salt.

 removes protein that is chemically joined to DNA

DNA will precipitate (form) in less dense ethanol layer

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