Chromatographic Methods in Pharmaceutical Analysis

Which type of chromatography was first developed by Mikhail Tsvet between 1901 and 1905?

• Gas chromatography

• Thin layer chromatography

• Column chromatography

• Liquid chromatography

What is the general principle behind the separation of compounds in chromatography?

• Differences in molecular weight

• Different interactions of analytes with stationary and mobile phases

• Differences in sample volume

• The color of the compounds

Which of the following correctly distinguishes between adsorption and partition chromatography?

• Adsorption uses a liquid stationary phase, while partition uses a solid stationary phase

• Adsorption involves the analyte dissolving into the mobile phase, while partition involves surface binding

• Adsorption chromatography is based on surface interaction with a solid stationary phase, while partition chromatography involves distribution between two liquid phases

• Both techniques use the same mechanism of separation

Which of the following is an example of planar chromatography?

• Column chromatography

• Gas chromatography

• TLC chromatography

• Ion exchange chromatography

In chromatography, when compounds are eluted first, it indicates that they:

• Have a strong interaction with the stationary phase

• Have a high affinity for the mobile phase

• Are highly volatile

• Are unable to pass through the mobile phase