Chromatography Quiz

What is the main purpose of chromatography?

• Generating electricity

• Building structures

• Creating chemical reactions

• Separation of components in a mixture

Answer explanation

The main purpose of chromatography is the separation of components in a mixture.

Which chromatography technique is commonly used for the purification of biomolecules?

• Paper chromatography

• Gas chromatography

• Column chromatography

• Thin-layer chromatography

Answer explanation

Column chromatography is commonly used for the purification of biomolecules due to its ability to separate compounds based on size, charge, and affinity.

What is the stationary phase in chromatography?

• A solid or liquid phase that moves with the aid of a mobile phase

• A gaseous component that separates molecules

• A layer of liquid adsorbed on the surface of a solid support

• A mobile phase composed of liquid

Answer explanation

The stationary phase in chromatography is a layer of liquid adsorbed on the surface of a solid support.

Which chromatography technique is used for the purification of enzymes, hormones, and specific proteins?

• Dye-ligand chromatography

• Affinity chromatography

• Gas chromatography

• Thin-layer chromatography

Answer explanation

Affinity chromatography is the chromatography technique used for the purification of enzymes, hormones, and specific proteins by exploiting the specific interactions between the target molecules and the stationary phase.

What is the basic principle of gel-permeation chromatography?

• Separation based on color

• Separation based on molecular sizes

• Separation based on charge

• Separation based on shape

Answer explanation

The basic principle of gel-permeation chromatography is separation based on molecular sizes, allowing smaller molecules to pass through the gel matrix more easily than larger ones.