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Chromatography Concepts and Comparisons

Authored by Rajapandiyan Panneerselvam

Chemistry

12th Grade

Used 1+ times

Chromatography Concepts and Comparisons
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15 questions

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1.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What is the principle behind High Performance Liquid Chromatography (HPLC)?

HPLC combines all components into a single phase for analysis.

HPLC relies solely on temperature variations for separation.

HPLC is used to measure the pH of solutions.

HPLC separates components in a mixture based on their interactions with a stationary phase and a mobile phase.

2.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

List two common applications of Thin Layer Chromatography (TLC).

Analyzing pharmaceutical purity and monitoring chemical reactions.

Measuring pH levels in solutions

Identifying molecular weights of compounds

Determining boiling points of liquids

3.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Compare the resolution capabilities of HPLC and TLC.

TLC is more suitable for high-resolution applications than HPLC.

HPLC has higher resolution capabilities than TLC.

HPLC and TLC have the same resolution capabilities.

TLC has higher resolution capabilities than HPLC.

4.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What type of stationary phase is typically used in HPLC?

Alumina powder

Silica gel or polymer-based materials

Cellulose fibers

Metal oxides

5.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

Describe the mobile phase used in Thin Layer Chromatography (TLC).

The mobile phase in TLC is a gas that does not interact with the sample.

The mobile phase in TLC is a solid that remains stationary on the plate.

The mobile phase in TLC is a solvent or mixture of solvents that facilitates the movement of the sample up the TLC plate.

The mobile phase in TLC is a gel that prevents the movement of the sample.

6.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

What are some common detection methods used in chromatography?

Common detection methods in chromatography include UV-Vis spectroscopy, fluorescence detection, mass spectrometry, and refractive index detection.

Thin layer chromatography

Gas chromatography

High-performance liquid chromatography

7.

MULTIPLE CHOICE QUESTION

30 sec • 1 pt

How does the flow rate affect the separation in HPLC?

The flow rate has no impact on the separation process.

Flow rate only affects the temperature of the mobile phase.

The flow rate affects separation by influencing retention time and interaction with the stationary phase.

Higher flow rates always improve resolution in HPLC.

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