Microscopy Quiz

The statements below are about light microscopes. 1 As the magnification increases the resolution decreases. 2 The resolution of a light microscope is limited by the wavelength of light. 3 To calculate the magnification of a light microscope the eyepiece lens and objective lens magnifications are added together. 4 The scale on a stage micrometer is resolved more clearly than an eyepiece graticule. Which statements are correct?

• A.1 and 2

• B.1 and 4

• C. 2 and 3

• D. 3 and 4

Which row compares correctly the magnification and resolution of an electron microscope with a light microscope?

The following statements describe drawing a scientific drawing. Which is not a correct statement about drawing scientifically?

• A. The magnification should be stated.

• B. Label lines should have arrowheads.

• C. White unlined paper should be used.

• D. Lines should be drawn with a smooth continuous line.

Compared to a light microscope the electron microscope has a higher resolution. Which of the following is a result of a higher resolution?

• A. The ability to see mRNA in all cells.

• B. The ability to see the nucleus in eukaryotes.

• C. The ability to produce larger images of cells.

• D. The ability to see cristae in mitochondria.

Cells can be measured using eyepiece graticules and stage micrometers. Which of the following correctly describes why a stage micrometer is calibrated?

• A. The eyepiece graticule is magnified by the objective lens.

• B. An eyepiece graticule can be used to make measurements.

• C. An eyepiece graticule magnifies the specimen.

• D. An eyepiece graticule makes comparisons.

A lysosome has a diameter of 0.4 µm. What is the diameter in nm?

• A. 4nm

• B. 40nm

• C. 400nm

• D. 4000nm

The electron micrograph below shows the organelles in a leaf cell. A ruler has been added to show the size of the image alongside the scale bar which shows the distance that represents 5 µm. What is the magnification of this image?

• A. x7000

• B. ×3570

• C. x2800

• D. x280

The electron micrograph below shows a root vascular system. The magnification of the image is x200 and Y has a diameter of1 cm when measured using a ruler. What is the diameter of the cell labelled Y?

• A.100 µm

• B. 50 µm

• C.10 µm

• D. 5 µm

The electron micrograph ofthe plant cell has a 2 cm scale line labelled 5 µm. What is the magnification?

The following statements describe slide preparation. Which one would refer to a wet mount preparation of a slide for observation with a light microscope?

• A. Methylene blue is added to the specimen.

• B. A coverslip is placed on the specimen.

• C. The low power lens is above the object on the stage.

• D. A drop of water is added to the specimen.