Microbiology Quiz

What is the purpose of using a capsule stain?

• To identify the presence of a protective layer around bacteria

• To measure the size of microorganisms

• To enhance the resolution of a microscope

• To differentiate between gram-positive and gram-negative bacteria

Which of the following is a use for darkfield microscopy?

• To observe live, unstained specimens

• To measure the wavelength of light

• To enhance the color of stained specimens

• To compare the size of different microorganisms

What is the main difference between an acidic dye and a basic dye?

• Acidic dyes stain the background, while basic dyes stain the cells

• Acidic dyes are used for gram-positive bacteria, while basic dyes are for gram-negative

• Acidic dyes enhance resolution, while basic dyes enhance magnification

• Acidic dyes are used in electron microscopy, while basic dyes are used in light microscopy

What is one use for the TEM (Transmission Electron Microscope)?

• To view the internal structure of cells

• To measure the speed of light

• To observe the surface of a specimen

• To compare different types of stains

What is the primary function of a phase-contrast microscope?

• To enhance contrast in transparent specimens

• To measure the refractive index of materials

• To magnify the color of stained samples

• To differentiate between living and dead cells

Which staining technique is used to identify acid-fast bacteria?

• Gram staining

• Ziehl-Neelsen staining

• Capsule staining

• Negative staining

What is the main advantage of using fluorescence microscopy?

• To observe the natural color of specimens

• To detect specific proteins or structures within cells

• To measure the thickness of cell walls

• To compare the density of different tissues

Which technique is used to visualize the surface of a specimen at high resolution?

• Scanning Electron Microscopy (SEM)

• Phase-contrast Microscopy

• Fluorescence Microscopy

• Darkfield Microscopy

What is the primary use of Gram staining in microbiology?

• To differentiate between gram-positive and gram-negative bacteria

• To measure the size of bacterial cells

• To enhance the visibility of viruses

• To identify the presence of a capsule

Which microscopy technique is best suited for observing live cells without staining?

• Phase-contrast Microscopy

• Scanning Electron Microscopy (SEM)

• Fluorescence Microscopy

• Brightfield Microscopy