Exploring Restriction Enzymes and Their Applications

The video tutorial explains the discovery and function of restriction enzymes, which are crucial in genetic engineering. It covers how these enzymes recognize specific DNA sequences and cut DNA at precise locations, resulting in either blunt or sticky ends. The tutorial also discusses the application of restriction enzymes in creating recombinant DNA and the process of separating DNA fragments using gel electrophoresis.

In what year were the two enzymes responsible for restricting the growth of bacteriophage in E. coli isolated?

• 1983

• 1973

• 1963

• 1953

What is the main difference between exonucleases and endonucleases?

• Exonucleases cut DNA at specific positions within the DNA.

• Exonucleases remove nucleotides from the ends of the DNA.

• Endonucleases add methyl groups to DNA.

• Endonucleases remove nucleotides from the ends of the DNA.

What is a recognition sequence in the context of restriction endonucleases?

• A sequence of 6 base pairs recognized by the enzyme.

• A sequence of 9 base pairs recognized by the enzyme.

• A sequence of 3 base pairs recognized by the enzyme.

• A sequence of 12 base pairs recognized by the enzyme.

Which of the following is an example of a restriction enzyme?

• Helicase

• RNA polymerase

• DNA ligase

• EcoRI

What does a palindromic nucleotide sequence mean?

• A sequence that reads the same in both directions.

• A sequence that reads differently in one direction.

• A sequence that reads differently in both directions.

• A sequence that reads the same in one direction.

What type of DNA ends are generated by staggered cuts?

• Blunt ends

• Flush ends

• Even ends

• Sticky ends

What is the role of DNA ligases in genetic engineering?

• To separate DNA fragments by size.

• To join DNA fragments with sticky ends.

• To add methyl groups to DNA.

• To cut DNA at specific sequences.

Why is the stickiness of DNA ends important?

• It prevents the DNA from being cut.

• It facilitates the action of DNA ligases.

• It helps in the addition of methyl groups.

• It makes the DNA more stable.

What is the purpose of gel electrophoresis in DNA analysis?

• To separate DNA fragments by size.

• To add methyl groups to DNA.

• To cut DNA at specific sequences.

• To join DNA fragments with sticky ends.

What is the process of extracting separated DNA bands from agarose gel called?

• Methylation

• Elution

• Electrophoresis

• Ligation