Microscope Operation and Techniques

This video tutorial covers the basics of microscopy, focusing on the preparation of temporary mounts and the use of optical microscopes. It explains how to prepare a sample, apply stains, and use a cover slip. The video also details the setup of an optical microscope, including its components and how to focus it. Additionally, it provides guidance on calculating magnification and using the microscope to observe prepared slides.

What type of microscope is NOT covered in this video?

• Fluorescence Microscope

• Electron Microscope

• Compound Microscope

• Optical Microscope

What is the first step in preparing a temporary mount?

• Using the fine adjustment wheel

• Preparing a thin slice of the sample

• Placing the cover slip

• Adding a drop of stain

Why is it important to add a drop of liquid to the sample?

• To enhance the color of the sample

• To make the sample thicker

• To bind to specific structures within the cell

• To prevent the sample from drying out

What is the purpose of a cover slip?

• To adjust the light intensity

• To provide a stable base

• To avoid trapping air bubbles

• To magnify the sample

What part of the microscope controls the amount of light passing through the sample?

• Objective lens

• Stage

• Eyepiece lens

• Diaphragm

Which lens should you start with when focusing a microscope?

• Times 40 objective lens

• Times 10 objective lens

• Times 4 objective lens

• Times 5 eyepiece lens

What is the function of the coarse adjustment wheel?

• To adjust the light intensity

• To change the objective lens

• To make tiny changes to the focus

• To move the stage up and down significantly

How do you avoid damaging the slide and lens when focusing?

• By using the fine adjustment wheel first

• By using a higher powered objective lens

• By lowering the stage while looking through the eyepiece

• By raising the stage as high as it goes while looking at the stage

How do you calculate the total magnification of an optical microscope?

• By adding the magnifications of the objective and eyepiece lenses

• By multiplying the magnifications of the objective and eyepiece lenses

• By subtracting the magnification of the eyepiece lens from the objective lens

• By dividing the magnification of the objective lens by the eyepiece lens

What is the total magnification if you use a times 5 eyepiece lens and a times 10 objective lens?

• Times 15

• Times 50

• Times 5

• Times 10