Cell Biology Quiz

You have homogenized plant tissue and would like to separate chloroplasts from nuclei. Which of the following methods would be most suitable?

• Polyacrylamide gel electrophoresis

• differential centrifugation using sucrose gradients

• equilibrium density gradient centrifugation on CsCl gradients

• gel filtration

Movement of substances across cell membrane is controlled by the

• size of permeating particles

• permeability of membrane

• membrane proteins

• all of the above

Diffusion across the plasma membrane is more rapid if a substance is

• a protein

• hydrophilic

• high in its oil: water partition coefficient

• large and globular in shape

Membranes of the following two organelles are contiguous

• ER and golgi

• nucleus and ER

• golgi and plasma membrane

• golgi and lysosomes

Which of the following are functions of RER?

P. Splicing of polypeptide

Q. formation of disulfide bond

R. glycosylation of proteins during translation

S. assembly of multisubunit proteins

• P and Q 

• R and S  

• P, Q and R  

• P, Q, R and S

A cell, which is very active in the synthesis and secretion of proteins, would be expected to have

• equal amount of SER and RER

• more rough than SER

• more smooth than RER

• neither SER and RER

Golgi complex plays a major role in

• protein synthesis

• glycosylation of proteins and lipids

• removal of sulfate from the carbohydrate moiety of glycolipids

• formation of secondary lysosomes

The major functions/features of the golgi apparatus in eukaryotic cells are

• it carries out glycosylation of the proteins being transported

• it is the major protein sorting centre of the cell

• it forms secretory vesicles in its trans compartment

• all of the above

A distinctive feature of the lysosome is that it has

• a lower pH than the cytoplasm

• a reduced hydrolase activity

• DNA

• ribosome

Which of the following enzymes do not occur in the lysosomes?

• phosphatase

• lipase

• proteases

• polymerase