REVISION WEEK 8

What is recombinant DNA technology?

• A process that only occurs in bacteria.
• Recombinant DNA technology is a method used to join together DNA from different organisms to produce desired traits.

• A method for editing existing DNA sequences.
• A technique to clone entire organisms.

The enzyme that joins foreign DNA fragments with the cloning vector to form recombinant DNA is called ___________.

Which of the following is NOT a tool used in recombinant DNA technology?

•  Target DNA

• Cloning vector

• DNA polymerase

• Host cell

 Name one example of a restriction enzyme used in recombinant DNA technology.

Which type of vector can carry the largest amount of foreign DNA?

• Plasmid

• Bacteriophage

• Cosmid

• Yeast artificial chromosome (YAC)

Draw a plasmid used in recombinant DNA technology and label the following parts:

• Origin of replication (ori)

• Selectable marker (antibiotic resistance gene)

• lac Z
• Multiple cloning site (MCS)

• Gene of interest (insulin gene)

 Illustrate the process of cleaving a plasmid using the EcoRI restriction enzyme 

The method used to determine if a bacterial colony contains the recombinant plasmid is called __________.

List one application of recombinant DNA technology.

What is the primary purpose of gene cloning in bacteria?

• To create genetically modified animals

• To produce proteins like insulin

• To induce mutations in plants

• To create new species