Flow Cytometry Overview Workshop

What technology provides rapid multi-parametric analysis of single cells in solution?

• Flow Cytometry

• Microscopy

• Spectrometry

• Centrifugation

What are the two types of light scatter measured in flow cytometry?

• Forward scatter and side scatter

• Fluorescent scatter and light scatter

• Visible scatter and invisible scatter

• None of the above

What is the purpose of fluorescent reagents in flow cytometry?

• To analyze cell populations

• To purify cells

• To provide light signals

• All of the above

What is the main function of a cell sorter in flow cytometry?

• To analyze cells

• To purify and collect samples

• To measure light scatter

• To transfect cells

What type of cytometer uses ultrasonic waves for better focusing?

• Acoustic Focusing Cytometer

• Traditional Flow Cytometer

• Mass Cytometer

• Imaging Cytometer

What is the role of dichroic filters in flow cytometry?

• To pass specific wavelengths of light

• To generate light signals

• To analyze cell populations

• To collect data

What is the main advantage of imaging flow cytometers?

• They combine flow cytometry with fluorescence microscopy

• They are cheaper than traditional cytometers

• They require less sample preparation

• They can only analyze single cells

What is the primary detection method used in mass cytometers?

• Time-of-flight mass spectrometry

• Fluorescent detection

• Light scatter detection

• Immunostaining

What is the purpose of MHC multimers in antigen specific responses?

• To bind to T cells that recognize the antigen

• To stimulate immune cells

• To measure cytokine production

• To analyze cell proliferation

What is the role of Annexin V in apoptosis detection?

• To bind to phosphatidylserine

• To label DNA breaks

• To measure mitochondrial potential

• To stain for viability