Biophysical JC4

Which of the following is false about LMW-E?

• 1. It is formed from FL-cycE

• 1. It accumulates in the nucleus

• 1. It is expressed by 70% of TNBCs

• 1. It has a greater affinity for CDK2 than FL-cycE

Which of the following is true about RP-6306 on apoptosis and cell cycle?

• 1. There was a significant increase in the LMW-E low cells going through apoptosis compared to untreated control cells

• 1. There was a significant decrease in the LMW-E low cells going through apoptosis compared to untreated control cells 

• 1. There was a significant decrease in the LMW-E high cells going through apoptosis compared to untreated control cells

• 1. There was a significant increase in the LMW-E high cells going through apoptosis compared to untreated control cells

Comparison of the induced expression of the two isoforms of cyclin E yielded which of the following discoveries?

• 1. FL-cycE, but not LMW-E, upregulates PKMYT1 and CDK1 pT14 levels

• 1. LMW-E only upregulated CDK2 pT160 and RB pS807/811

• 1. LMW-E, but not FL-cycE, upregulates PKMYT1 and CDK1 pT14 levels

• 1. FL-cycE, but not LMW-E, upregulates CDK2 pT160 and RB pS807/811

What is true of tumor sizes after RP-6306 treatment in high VS low LMW-E expressing tumors?

• High and low LMW-E tumors BOTH experienced a significant growth delay when treated with RP-6306

• Only high LMW-E tumors experienced a significant growth delay when treated with RP-6306

• Only low LMW-E tumors experienced a significant growth delay when treated with RP-6306

• Neither high nor low LMW-E tumors experienced a significant growth delay when treated with RP-6306

Where are FL-cycE and LMW-E accumulated in the cell?

• a) FL-cycE in the cytoplasm, LMW-E in the nucleus 

  
  

• b) LMW-E in the cytoplasm, FL-cycE in the nucleus

  
  

• c) Both in the nucleus 

  
  

• d) Both in the cytoplasm 

  
  

What is one way that enhances LMW-E’s potential to regulate PKMYT1?

• PKMYT1 localizes with LMW-E in the nucleus

• PKMYT1 localizes with both LMW-E and FL-cycE

• PKMYT1 localizes with FL-cycE in the nucleus

• PKMYT1 localizes with LMW-E in the cytoplasm

Who here didn't do the assignment??????