Chapter 3: Blood Bank Applications of Antigen-Antibody Reactions

Which tests utilize the AHG technique?

• Antibody screen

• Antibody Identification

• Crossmatch

• All of the above

IgG antibodies are detected by the use of which reagent?

• LISS

• PEG

• AHG

• Check Cells

The test that detects antibodies bound to RBCS in vivo is the:

• IAT

• DAT

• Antibody screen

• Antibody Identification

For the DAT, anti-IgG and anti-C3d are part of the _______ reagent.

• Polyspecific

• Monospecific

What must be added to all tubes that give a negative reaction at the AHG phase of testing?

• LISS

• PEG

• Check Cells

• Nothing

The purpose of enhancement reagents is:

• increase incubation time

• increase rate and specificity of antibody attachment

• influence lattice formation

• increase supplier cost

Which class of antibodies react best at room temperature?

• IgG

• IgA

• IgM

• IgE

If no agglutination is seen at the 37, AHG, or Check Cell phases of testing, what is the MLT's next step?

• Report as positive

• Report as negative

• Repeat test

• Add LISS

In solid phase testing, a solid pellet of cells indicates a ____ reaction.

• Positive

• Negative

In gel testing, a solid band on top of the gel indicates a _____ reaction.

• Positive

• Negative