Microscope Quiz

What is magnification?

• Ability to distinguish two points as separate

• Difference in light intensity between the image and background

• Enlarging the appearance of an object

• Controlling the amount of light passing through the specimen

What is the total magnification if the objective lens is 40x and the ocular lens is 10x?

• 50x

• 400x

• 100x

• 4x

Which property refers to the ability to distinguish two points as separate?

• Contrast

• Magnification

• Resolution

• Depth of Field

What type of light is preferred for higher resolution in microscopy?

• Longer wavelength

• Shorter wavelength

• Infrared light

• Visible light

How does increasing the numerical aperture (NA) affect resolution?

• Decreases resolution

• Increases resolution

• Does not affect resolution

• Only affects magnification

What is contrast in microscopy?

• The amount of light entering the microscope

• Difference in light intensity between the image and the background

• The thickness of the specimen that remains in focus

• The magnification of the objective lens

What technique can improve contrast when viewing biological samples?

• Magnification

• Staining

• Coarse focusing

• Resolution

Which microscope uses visible light to illuminate samples?

• Scanning Electron Microscope (SEM)

• Compound Light Microscope

• Transmission Electron Microscope (TEM)

• Phase Contrast Microscope

As magnification increases, what happens to the field of view?

• Increases

• Stays the same

• Decreases

• Becomes 3D

What is depth of field in microscopy?

• The number of lenses used in the microscope

• The total magnification of the specimen

• The thickness of the specimen that remains in focus

• The amount of light that enters the microscope