EXAM 1

To determine AML from ALL, AML is positive and ALL is negative

• Sudan Black

• Myeloperoxidase

• Sudan Black and Myeloperoxidase

• PAS

What does myeloperoxidase stain?

• primary granules

  of neutrophils

• Secondary granules of neutrophils

• Myelocytic cells

• Blast cells

Myeloperoxidase, sudan black, and PAS cells reviewed for reaction are

• Blast cells

• Monocytic cells

• Monocytes and neutrophils

• Eosinophils

What does Sudan Black stain?

• Lipids in granules

  of neutrophils

• Lipids in myelocytic cells

• Lipids in primary granules of netrophils

• Lipids in secondary granules on neutrophils

Used for myelocytic cells (Positive)

• Chloroacetate

  Esterase

• Alpha-Naphthyl

  Butyrate/Alpha-

  Naphthyl

  Acetate Esterase

• Sudan Black

• LAP

Used for Monocytic cells (Positive)

• Alpha-Naphthyl

  Butyrate/Alpha-

  Naphthyl

  Acetate Esterase

• Chloroacetate

  Esterase

• PAS

• TRAP

Stains Esterase

Isoenzymes

• Chloroacetate

  Esterase

• Chloroacetate

  Esterase and Alpha-Naphthyl

  Butyrate/Alpha-

  Naphthyl

  Acetate Esterase

• LAP

• TRAP

Alpha-Naphthyl
Butyrate/Alpha-
Naphthyl
Acetate Esterase cells reviewed for reaction are

• Monocytes and neutrophils

• Monocytes

• Myelocytic cells

Cells reviewed for Chloroacetate

Esterase are

• Neutrophilic cells

• Neutrophils and monocytes

• Myelocytic cells

• Lymphocytes

What does PAS stain?

• Glycogen

• Isoenzyme 5

• Lipids in granules and neutrohils

• enzyme found in

  primary granules

  of neutrophils