Sequencing 2024 Quiz

What are the two methods in basic sequencing techniques?

• Chemical Termination method & Sanger method

• Pyrosequencing method & Sanger method

• Dideoxy sequencing & Chemical Degradation method

• Maxam-Gilbert method & Shotgun sequencing

Piperidine is used to cut...

• Glycosidic bond

• Phosphodiester bond

• Ribose bond

• Hydrogen bond

What is the specific format used to copy our sequence into BLAST?

• NGS

• MEGA

• FASTA

• SEQ

What will dimethyl sulfate methylate in an acidic environment?

• Cytosine

• Guanine

• Pyrimidine

• Purine

When setting up a Sanger sequencing reaction, each reaction should include template DNA, nucleotides, dideoxynucleotides, buffer, DNA polymerase, and _____.

• Forward AND reverse probes

• Forward AND reverse primers

• Forward OR reverse primers

• Forward OR reverse probes

What is the sequence of the DNA fragment from the image?

Automated DNA sequencing reactions are done in a single tube because each dNTP is labeled with a different fluorescent dye.

• FALSE

• TRUE

In the Sanger method, we adopt the PCR technique and electrophoresis with agarose gel to amplify the DNA template and separate the fragments, respectively.

• TRUE

• FALSE

Which one of the molecules below is the ddNTP molecule?

• 
• 
• 
• 

In the Maxam-Gilbert method, the chemicals used for identifying Cytosine and Thymine are….

• Hydrazine + piperidine

• Dimethyl sulphate in formic acid + piperidine

• Hydrazine in NaCl + piperidine

• Dimethyl sulphate + piperidine