Lecture 16 Flow cytometry

The difference between a humanized and chimeric antibody is that the humanized only contains the CDRs from the original host antibody while the chimeric contains the the variable regions from the original host antibody.

• True

• False

An antibody made by immunizing a mouse may no longer bind to human Fc receptors.

• True

• False

Which of the following cell populations are double negative T cells (CD4-, CD8-)?

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A monoclonal antibody is produced by collecting antibodies from different B cells clones that bind to different epitopes within the same antigen.

• True

• False

Virus X is hypothesized to infect a specific subtype of T cells. You decide to infect a mouse and isolate all the cells from the lymph node. If infected, the cell should express the viral antigen Y. You decide to stain the sample using the following monoclonal antibodies: Y - viral antigen, CD3, CD4, IL-17 (cytokine produced by Th17 subtype . Based on the provided dot blots, which of the following statements are true?

• the total number of T cells in the sample is 22%

• Virus X infects Th17 cells

• Virus X can infect other cells aside from T helper cells

• Virus X seems to replicate better in cells other than T helper cells

• Only Th cells express CD4

Which of the following statements is true about polyclonal antibodies?

• They are produced by a single clone of B cells.

• They recognize multiple epitopes on the same antigen.

• They are identical in structure and function.

Which of the following is a potential drawback of using chimeric antibodies in human therapy?

• They are less specific than humanized antibodies.

• They may elicit an immune response due to their non-human components.

• They cannot be produced in large quantities.

• They are unable to bind to human antigens.

Which of the following cell populations are CD4 T cells (CD4+, CD8-)?

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