Exploring PCR Techniques and Applications

What are the main steps involved in the PCR process?

• Denaturation, Annealing, Extension

• Separation, Combination, Amplification

• Replication, Transcription, Translation

• Initiation, Elongation, Termination

List three applications of PCR in scientific research.

• Plant growth enhancement

• Nutrient extraction

• Water purification

• 1) Genetic cloning, 2) Disease diagnosis, 3) Forensic analysis

What are the different types of PCR techniques?

• Standard PCR, Real-Time PCR (qPCR), Reverse Transcription PCR (RT-PCR), Multiplex PCR, Nested PCR, Digital PCR, Hot Start PCR.

• Gel Electrophoresis

• Sanger Sequencing

• Polymerase Chain Reaction (PCR)

How is PCR utilized in forensic science?

• PCR is used to amplify DNA for analysis in forensic investigations.

• PCR is utilized to analyze blood types in forensic cases.

• PCR helps in the identification of fingerprints.

• PCR is used to create physical evidence at crime scenes.

Explain the significance of PCR in genetic testing.

• PCR is significant in genetic testing because it amplifies DNA, allowing for the detection of genetic mutations and analysis of small samples.

• PCR can only analyze large amounts of DNA.

• PCR is primarily used for protein synthesis in cells.

• PCR is used to sequence RNA directly from samples.