CRISPR Review
Flashcard
•
Biology
•
11th - 12th Grade
•
Hard
Wayground Content
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15 questions
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1.
FLASHCARD QUESTION
Front
What is a PAM in the context of CRISPR?
Back
A PAM (Protospacer Adjacent Motif) is a 3 bp sequence that is adjacent to viral DNA in a CRISPR array.
2.
FLASHCARD QUESTION
Front
What type of DNA repair mechanism creates a mutation and inactivates the gene after Cas9 cuts the target DNA?
Back
Non-Homologous End Joining (NHEJ) is the repair mechanism that creates a mutation and inactivates the gene.
3.
FLASHCARD QUESTION
Front
Provide an example of a PAM sequence.
Back
AGG is an example of a PAM sequence.
4.
FLASHCARD QUESTION
Front
What is the role of guide RNA (gRNA) in the CRISPR-Cas9 mechanism?
Back
The guide RNA (gRNA) binds to the target DNA sequence and guides the Cas9 enzyme to the correct location.
5.
FLASHCARD QUESTION
Front
What does the acronym CRISPR stand for?
Back
CRISPR stands for Clustered Regularly-Interspaced Short Palindromic Repeats.
6.
FLASHCARD QUESTION
Front
What is the function of the Cas9 enzyme in the CRISPR system?
Back
The Cas9 enzyme functions as a molecular scissors that cuts the target DNA at a specific location.
7.
FLASHCARD QUESTION
Front
How does CRISPR technology differ from traditional genetic modification methods?
Back
CRISPR technology allows for precise editing of specific genes, whereas traditional methods often involve random insertion of genes.
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