Chromatography Part 1

Which phase is static in chromatography?

• mobile phase

• stationary phase

• none

Chromatography is all about?

• mixing of mixture

• mobile phase and stationary phase

• separation of mixture

Choose the examples of column chromatography

• Paper and TLC

• Paper and GC

• GC and HPLC

• TLC and GC

Formula for retention factor, K'

• k' = tr - tm/tm

• alpha = k' A / k' B

In adsorption chromatography, stationary phase is a polar solid. Therefore, polar substance will

• stay in place

• less adsorb than less polar molecule

• more adsorb than less polar molecule