Determination thiocyanate in waste water using UV-Vis spectro

What are the aims of experiment this week? (multiple answers)

• to determine the concentration of SCN- in unknown sample

• To determine the maximum wavelength of SCN- ions

• to prepare a series of standard solutions of thiocyanate

• To observe the interaction of thiocyanate ions with UV-light

At which wavelength do you need to measure the absorbance of SCN- ions using UV-Vis?

Why do you need to measure absorbance at an optimum wavelength? (multiple answers)

• To give maximum correctness

• to ensure maximum sensitivy

• optimum wavelength correlates to the largest response of analyte

• To minimize errors of measurement

Which statements are correct? (multiple correct answers)

• The absorbance is directly proportional to concentration of sample

• Depends on molar extinction coefficient of substance

• Wavelength is directly proportional to absorbance

• The absorbance is directly proportional to the length of light path (width of cuvette)

Fill in the following substances for your experiment this week:

a. Analyte: ..............

b. Standard solutions: ..............

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