PCR Experiment Quiz (Antibiotic Resistance Lab)

How many PCR tubes should you labeled before you start the lab?

• 2

• 3

• 4

• 5

What is the label for the tube containing the Negative Control?

• Tube P

• Tube N

• Tube A

• Tube B

What is the purpose of tapping the tubes on the bench?

• To remove air bubbles

• To collect liquid at the bottom

• To mix the reagents

• To cool the samples

How many microliters of liquid will each PCR tube have once you pipette all of the reagents?

• 10

• 15

• 25

• 20

What is the annealing temperature?

• 94

• 55

• 75

• 98

How many cycles should you set your thermocyclers to?

• 30

• 28

• 35

• 32

True or False: The heat lid should be on for this lab.

• True

• False

How much ladder should you load into the first well?

• 15

• 12

• 10

• 8

What indicates that the PCR run has completed on the miniPCR® thermal cycler?

• The device shuts down

• The app status shows "Finished" and LEDs light up

• The protocol restarts

• The device beeps

What percentage of agarose should be your gel?

• 2.5%

• 2%

• 1%

• 1.5%