Quiz on Cryopreservation of Cultured Animal Cells

What is the primary purpose of cryopreservation?

• To promote cell death

• To increase cell differentiation

• To preserve living cells and tissues

• To enhance cell growth

Which factor is crucial for maximizing cell viability upon thawing?

• Increasing cell concentration

• Rapid cooling

• Minimizing intracellular ice crystal formation

• Using non-penetrating cryoprotectants

What is the best cryoprotectant mentioned for freezing cells?

• Trehalose

• Glycerol

• PEG

• DMSO

What is a significant risk associated with rapid cooling of cells?

• Improved membrane function

• Enhanced cryoprotectant efficacy

• Cold shock damage

• Increased cell growth

What is the recommended cooling rate for controlled freezing?

• 1°C/min

• 5°C/min

• 10°C/min

• 0.5°C/min

Which type of cryoprotectant does not cross the cell membrane?

• DMSO

• Trehalose

• Polyvinylpyrrolidone

• Glycerol

What is the main concern when transporting frozen ampoules?

• Oxygen exposure

• Light exposure

• Cell growth

• Temperature fluctuations

What should be done to minimize genetic drift in cell cultures?

• Increase cell concentration

• Replace stocks regularly

• Use more cryoprotectants

• Decrease freezing time

What is the purpose of using a hydrophilic cryoprotectant?

• To increase intracellular ice formation

• To enhance cell differentiation

• To sequester water

• To promote cell growth

Which of the following is a benefit of cryopreservation?

• Increased cell differentiation

• Generation of safety stocks

• Reduction of cell viability

• Enhanced cell metabolism