MI Midterm Mini-Quiz

You start with 1 mL of a 1 mg/mL solution and perform a 1:10 serial dilution by transferring 1 mL into 9 mL of diluent. You repeat this process four more times. What is the final concentration in the fifth tube?

Tags

NGSS.HS-PS1-7

A researcher performs a serial dilution starting with a stock solution of 1,000 ng/mL: Step 1: 1:2 dilution. Step 2: 1:5 dilution of Step 1. Step 3: 1:3 dilution of Step 2. What is the final concentration after all three steps?

After performing a 1:10 serial dilution across three steps, the concentration in the final tube is 0.01 ng/mL. What was the concentration of the original stock solution?

A new ELISA test is developed for detecting a protein related to a rare disease. If the test line does not change color during the trial, what potential errors in the test design could explain this result?

• The antibodies used are not specific to the target protein.

• The substrate used is not reactive with the enzyme.

• The enzyme is inactive or denatured.

• All of the above.

During an ELISA experiment, a researcher prepares a serial dilution with an initial concentration of 100 ng/mL. If each step involves a 1:4 (1/4) dilution, what is the final concentration after three dilutions?

• 100 ng/mL

• 25 ng/mL

• 6.25 ng/mL

• 1.56 ng/mL

What does a BLAST result with a high E value and low percent identity indicate about a pathogen's DNA, and what additional steps should be taken for pathogen identification?

• The DNA is a close match; proceed with treatment.

• The DNA is not a close match; further analysis is needed.

• The DNA is a perfect match; no further steps are required.

• The DNA is irrelevant; discard the results.

A company is engineering bacteria to produce insulin. What ethical considerations should be addressed before the product is released for public use?

• Safety and efficacy of the product

• Environmental impact of genetically modified bacteria

• Access and affordability of insulin

• All of the above

Tags

NGSS.HS-ETS1-3

NGSS.HS-LS2-7

What is the role of conjugation in antibiotic resistance development, and how could this process impact treatment plans for bacterial infections? Select the best statement that explains this:

• Conjugation allows bacteria to transfer genetic material, including antibiotic resistance genes, which can make infections harder to treat.

• Conjugation is a process where bacteria produce antibiotics to kill other bacteria, reducing resistance.

• Conjugation involves the mutation of bacterial DNA, leading to resistance without gene transfer.

• Conjugation is a method of bacterial reproduction that does not affect antibiotic resistance.

What type of hearing loss (if at all) does this patient have?

Tags

NGSS.HS-PS4-5

Why is it important to complete a prescribed course of antibiotics even after symptoms improve?

• To prevent antibiotic resistance

• To save money on medication

• To avoid side effects

• To reduce the dosage