Discussion-1-Jan 15, 2025

Which of the following is a soft ionization technique used in proteomics?

• Electron Impact (EI)

• Electrospray Ionization (ESI)

• Arc Discharge Ionization

• Laser Ablation

What is the primary purpose of a mass analyzer in mass spectrometry?

• Generate ions from biomolecules

• Separate ions based on their mass-to-charge ratio (m/z)

• Detect ions based on their fluorescence

• Fragment ions into smaller pieces

Which of the following mass analyzers provides the highest resolution and mass accuracy?

• Quadrupole

• Time-of-Flight (TOF)

• Orbitrap

• Magnetic Sector

What is the key advantage of hybrid mass spectrometers, such as Q-TOF or Q-Orbitrap?

• Faster ionization

• Integration of multiple analyzers for tandem MS/MS capabilities

• Reduction in sample preparation time

• Enhanced matrix compatibility

What is the purpose of MS/MS (i.e., MS2) in tandem mass spectrometry?

• Measure the m/z ratio of intact peptides

• Fragment peptides to determine their primary sequence

• Ionize peptides for analysis

• Separate proteins by their molecular weight