Biochemistry Practical Quiz

What are the main pieces of equipment listed for a biochemistry practical? What are their specific uses?

How does a volumetric flask differ from a measuring cylinder?

What is the function of phenolphthalein, and what pH range does it indicate?

How does methyl orange differ in its color change compared to phenolphthalein?

What is a buffer, and why is it important in biochemical experiments?

What two components form a buffer system? Provide an example from the notes.

Explain the concept of “buffer capacity.” How is it affected by the pH relative to the buffer’s pKa?

Describe the phosphate buffer system. How does it maintain pH balance?

Write out the Henderson-Hasselbalch equation and explain its components.

How can you use the Henderson-Hasselbalch equation to calculate the ratio of conjugate base to acid at a given pH?