Biochemistry Practical Quiz

What are the main pieces of equipment listed for a biochemistry practical? What are their specific uses?

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How does a volumetric flask differ from a measuring cylinder?

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What is the function of phenolphthalein, and what pH range does it indicate?

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How does methyl orange differ in its color change compared to phenolphthalein?

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What is a buffer, and why is it important in biochemical experiments?

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What two components form a buffer system? Provide an example from the notes.

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Explain the concept of “buffer capacity.” How is it affected by the pH relative to the buffer’s pKa?

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Describe the phosphate buffer system. How does it maintain pH balance?

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Write out the Henderson-Hasselbalch equation and explain its components.

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How can you use the Henderson-Hasselbalch equation to calculate the ratio of conjugate base to acid at a given pH?

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