Gel electrophoresis 1

Put the steps of gel electrophoresis in order:

• Electric current is applied

• DNA fragments are loaded on the negative charge ends. 

• DNA fragments move towards the positive end of the gel

• Smaller fragments move faster than larger fragments

Use the image to answer the next three questions:

1. 1. This lane contains the longest DNA fragment​ ​ (a)  

2. 2. This lane contains 1500 base pair (bp) DNA​ (b)  

3. 3. This lane contains the shortest DNA fragment​ (c)  

• 1
• 3
• 2
• 4
• DNA ladder

•Study the DNA samples and compare them with the puppy’s DNA (ladder). Which dog is the father of the puppy?

• A

• B

• C

• D

Study the results of the electrophoresis and try to determine the size of DNA fragments. Plus, label the +ve side and the -ve sides of the gel.

a

b

c

d

e

f

g

positive

330

100

negative

200

700

500

Who is the baby's biological father?

Study the DNA samples. Who is the suspect who's committed the crime?

Which two bat species are most closely related?

•  Bat 1 and Bat 2
• Bat 1 and Bat 3
• Bat 2 and Bat 3
• There’s no way to tell

Tags

NGSS.HS-LS4-1

The band with heavier fragment of DNA.

Note: Cathode means positive.