PCR Lab

denaturation

• a strand of DNA from which new DNA is synthesized

• Unique DNA banding pattern used for identity purposes

• separation of DNA strands by breaking hydrogen bonds

• source of As, Cs, Gs, and Ts during DNA replication

• determining the best conditions and concentrations for a reaction

DNA fingerprinting

• a strand of DNA from which new DNA is synthesized

• Unique DNA banding pattern used for identity purposes

• separation of DNA strands by breaking hydrogen bonds

• source of As, Cs, Gs, and Ts during DNA replication

• determining the best conditions and concentrations for a reaction

dNTPs

• a strand of DNA from which new DNA is synthesized

• unique DNA banding pattern used for identity purposes

• separation of DNA strands by breaking hydrogen bonds

• source of As, Cs, Gs, and Ts during DNA replication

• determining the best conditions and concentrations for a reaction

optimization

• a strand of DNA from which new DNA is synthesized

• unique DNA banding pattern used for identity purposes

• separation of DNA strands by breaking hydrogen bonds

• source of As, Cs, Gs, and Ts during DNA replication

• determining the best conditions and concentrations for a reaction

template

• a strand of DNA from which new DNA is synthesized

• unique DNA banding pattern used for identity purposes

• separation of DNA strands by breaking hydrogen bonds

• source of As, Cs, Gs, and Ts during DNA replication

• determining the best conditions and concentrations for a reaction

Tags

NGSS.HS-LS3-2

PCR improved older DNA fingerprinting methods because of all the following reasons except:

• it gives more reputable data

• pcr is faster

• it requires less starting DNA

• It can be used to compare closely related proteins

When replicating DNA in the lab, what is used to indicate the starting place for synthesis?

• Template

• Primer

• dNTPs

• DNA polymerase

PCR is useful for all of the following reasons except:

• targeting a specific region in a huge genome

• identifying proteins with site specific mutant amino acids

• identifying size differences between individuals at a particular locus

• making many copies of a particular gene segment

When using PCR for identifying purposes,

• highly conserved regions of the genome should be targeted

• highly variable regions of the genome should be targeted

• any area on the genome would work well

• making many copies of a particular gene segment

How many cycles does a PCR reaction usually go?

• 10-20

• 20-40

• 40-60

• 50-100