Plasmids and Biotechnology

What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into a bacterium?
I) Grow transgenic bacteria.
II) Cut the plasmid DNA using restriction enzymes.
III) Extract plasmid DNA from a bacterial cell.
IV) Create recombinant DNA.
V) Combine the gene of interest to the plasmid, creating recombinant DNA

• I, II, IV, III, V
• III, II, IV, V, I
• II, III, V, IV, I
• III, IV, V, I, II

How do EcoRI and BamHI differ?

• different blunt ends
• different restriction sites
• produce different proteins
• cut DNA 

A length of unpaired bases thatstick out.  These have been cut from a specific bases in a specific sequence of bases.  

• Sticky Ends 
• Recombinant DNA 
• DNA size standard
• blunt ends

What would have caused the plasmid (B) to open?

• restriction enzyme
• ligase
• gene therapy
• sticky ends

Which enzyme "glues" DNA fragments together?

• restriction enzymes
• DNA ligase
• DNA polymerase
• DNA helicase

What fragment would NOT be created by cutting this plasmid with HindIII?

• 3 kb
• 7 kb
• 4 kb
• all of the above

Sticky or blunt ends?

• sticky
• blunt

Which fragments would be formed by cutting this DNA with EcoRI?

• 5 kb, 5 kb
• 1.5 kb, 3.5 kb, 5 kb
• 1.5 kb, 5.5 kb, 3 kb
• none of the above

Which of the following results would indicate that transformation of the bacteria occurred?

• Growth in LB agar
  
• Growth in LB/ Amp agar by bacteria with the plasmid 
  
• Growth in Amp agar by bacteria without plasmid
• None of these answers are correct