Chromatography Thin Layer

TLC stands for...

• Tender Love & Care

• Thick Layer Chromatography

• Thin Layer Chromatography

• Thin Liquid Chromatography

The distance travelled by the compound depends on how well it adheres to the plate.

• TRUE

• FALSE

The distance travelled by the compound depends on how soluble it is in the chosen solvent.

• TRUE

• FALSE

How to calculate Rf?

• Rf = distance travelled by sample / distance travelled by solvent

• Rf = distance travelled by solvent / distance travelled by sample

When could a co-spot be used?

• to make a direct comparison on a TLC plate between the tested compound and the pure substance

• to improve the solubility of the tested compound in the solvent

• to increase the Rf value

• to decrease the Rf value

TLC can be used to_____. Tick all that apply

• assess the purity of substances

• confirm the identity of a compound

• separate a mixture of immiscible liquids

• purify a substance

_________________ utilizes a stationary and a mobile phase to separate mixtures based on their affinity for each phase.

• filtration
• chromatography
• distillation
• osmosis

The mobile phase in paper chromatography is typically a

• plasma
• solid
• gas
• liquid

The substance in the mixture that has the greatest affinity for the mobile phase will travel

• fastest
• slowest

The substance in the mixture that has the greatest affinity for the stationary phase will travel

• fastest
• slowest