Colony Transformation Lab

What is the circular piece of DNA found in bacteria called?

• homologous chromosome
• sister chromatid
• plasmid
• arabinose

Making changes in the DNA of an organism is

• selective breeding
• artificial selection
• genetic engineering
• genetic transformation

Which gene WAS NOT included in the +pGlo plasmid?

• antibiotic resistance gene
• green fluorescent protein gene
• hemophilia gene

What abbreviation represents the sugar molecule needed to "turn on" the green fluorescent protein gene?

• LB
• AMP
• ARA
• +pGlo

What is the name of the plasmid we used for lab?

• GFP

• ampR

• araC

• pGLO

Which gene is responsible for making the bacteria resistant to ampicillin?

• ampR

• pGLO

• araC

• GFP

 What was used to cut the plasmid to enable us to insert the desired gene?

• ligase
• restriction enzymes
• GFP
• pGLO

What did we use to create temperature differential to sweep plasmid inside

• heat shock
• ice
• arabinose
• CaCl₂

Which plate will have bacterial growth and bacterial GLOWth?

• LB

• LB/Amp

• LB/Amp/Ara

• All

What is used to ensure the bacteria transformed with the gene for antiobiotic resistance as only the transformed bacteria will grow on this medium?

• ampicillan
• arabinose
• LB
• GFP