PCR Review

What is the second step of PCR?

• denaturation

• extension

• annealing

• termination

• splicing

What does PCR accomplish?

• determining the sequence of DNA

• develop proteins

• make copies of a DNA section

• make mRNA

How many copies are made in PCR?

• 4

• 2

• over a billion

• 10000

How many cycles of PCR are typically done?

• 1-5

• 25-40

• 5-10

• 15-25

What is PCR used for? (choose all that apply)

• determining biological relationships

• identifying genetic mutations

• diagnosis of genetic diseases

• initiating cell differentiation

Tags

NGSS.HS-LS3-2

What is the denaturation step?

• mutating RNA polymerase

• Cooling DNA to allow primers to form

• separating DNA helix in a cold environment

• separating DNA helix with using heat

What process precedes PCR?

• cloning

• transformation

• gel electrophoresis

• meiosis

From where does Taq polymerase come?

• Bacteria that live in hydrothermal vents/hot springs.

• Cambodia

• The rain forest

• The CDC

What happens in the Denature step of PCR?

• The DNA nucleotides are broken apart.
• The base-pairing rules for DNA are reversed.
• The double-stranded DNA is separated into two single strands of DNA.
• The DNA is returned to its natural setting.

At what temperature does the Denature step of PCR occur?

• 95° C

• 50° C

• 72° C

• Any temperature