Recombinant Product

What is recombinant DNA technology?

• A method for creating new species

• The combination of DNA from different organisms to create new genetic material

• A process for cutting and rearranging protein molecules

• A method to replicate RNA molecules

Which restriction enzyme produces sticky ends?

• SmaI

• EcoRI

• HindIII

• BamHI

Restriction enzymes are also known as:

•  DNA ligases

• Endonucleases

• RNA polymerases

• Topoisomerases

Enzymes that recognize and cleave/cut DNA into DNA fragments at specific base sequences of DNA called ____________ site.

Recombinant DNA technology involves combining a gene of interest with a cloning __________ to form recombinant DNA.

Which of the following is a commonly used cloning vector?

• Plasmid

• Bacteriophage

• Cosmid

• YAC (yeast artificial chromosome)

Which enzyme is responsible for joining the gene of interest to the DNA cloning vector?

• Taq polymerase

• DNA ligase

• Restriction enzyme

• Helicase

What is the role of the host cell in recombinant DNA technology?

• To provide restriction enzymes

•  To replicate and express the recombinant DNA

• To bind the plasmid to the gene of interest

• To cut the plasmid at restriction sites