Bacterial Transformation Lab

What is transformation?

• The use of viruses to transform or genetically engineer cells.

• The measure of how well cells are transformed in a new phenotype.

• The insertion of a plasmid with a foreign gene into a bacterial cell which results in new acquired traits.

• Genetic engineering or transformation of mammalian cells.

When fragments of DNA are connected from different sources, this creates:

• A plasmid

• A clone

• Recombinant DNA

• Fragmented DNA

Bacterial DNA that is often circular or in the shape of a ring is called a:

• Clone

• Plasmid

• Fragment

• Recombinant DNA

Organisms that have had DNA from other organisms inserted into their DNA to produce certain traits are called:

• Plasmids

• Clones

• Transgenic organisms

• Recombinant organisms

Which of the following is a medical application of genetic engineering?

• Inserting human DNA into bacterial DNA for insulin production

• Manipulating of DNA to produce glowing monkeys

• Engineering crops for greater nutritional content

• Modifying genes to achieve a desirable phenotype

Which of the following means of genetic engineering would have the greatest impact on world hunger?

• Inserting human DNA into bacterial DNA for insulin production

• Manipulating DNA in trees to require less water

• Engineering crops for greater nutritional content

• Modifying genes to achieve a desirable phenotype

Tags

NGSS.HS-ETS1-3

NGSS.HS-LS3-2

Inserting human DNA into a bacterial plasmid to create insulin-producing bacteria is an example of:

• Recombinant DNA

• Bacterial Transformation

• Cloning

• Selective breeding

Crops are often genetically engineered for improved traits. Such crops and organisms are called:

• Genetically modified organisms

• Clones

• Transformed

• Nutritionally improved

DNA can be cut with

• ligase

• helicase

• restriction enzymes

• DNA scissors

A scientist wants to combine a bacterial plasmid with a human insulin gene.

• She should use the same restriction enzyme to cut them to make blunt ends

• She should use the same restriction enzyme to cut them to make sticky ends

• She should use different restriction enzyme to cut them to make blunt ends

• She should use different restriction enzyme to cut them to make sticky ends