Sanger Sequencing

A technique for making millions of copies of a specific region of DNA.

• DNA ligase

• restriction enzymes

• gel electrophoresis

• polymerase chain reaction

What is the function of a primer?

• To create a start point for copying.

• To copy DNA.

• To create DNA nucleotides.

• To identify which nucleotide is which.

What is the function of DNA Polymerase?

• attach primers to target sequence

• separate the DNA

• attach nucleotides to the target sequence

• separate the fragments according to size

How is DNA synthesis in PCR and Cycle Sequencing DIFFERENT?

• Only PCR uses deoxynucleotides.

• Cycle Sequencing generates DNA products of same lengths.

• Only PCR analyzes DNA products using gel electrophoresis.

• Only Cycle Sequencing uses dideoxynucleotides.

Larger DNA fragments move through a gel _____________ smaller DNA fragments.

• faster than

• at the same rate as

• slower than

• much more faster than

What is it called when the order of DNA nucleotides is determined for a section of DNA?

• DNA sequencing

• Denaturation

• Gel Electrophoresis

• DNA replication

Chain-termination is a type of_____

• Sequencing

• Vector generation

• Antibiotic production

• Gene manipulation

What is the main enzyme component of Sanger sequencing

• Helicase

• Polymerase

• Nuclease

• Gyrase