DNA Replication with Enzymes

Enzyme that unzips the DNA to prepare for replication

• polymerase III

• helicase

• topoisomerase

• ligase

To prevent supercoiling, which enzyme would play it role?

• Single-strand protein

• Primase

• Topoisomerase

• Polymerase I

What enzyme will solve this problem in lagging strand?

• Ligase

• SSB protein

• Helicase

• Polymerase I

__________ prevent the reformation of double helix

• SSB protein

• Polymerase I

• Gyrase

• Ligase

How many enzymes involved in DNA replication?

• 5

• 7

• 6

• 8

The synthesise of DNA by DNA polymerase occurs in

• 3' to 5' direction

• 5' to 5' direction

• 5' to 3' direction

• 3' to 3' direction

What enzyme replaces RNA primer on the leading strand with DNA?

• Polymerase I

• Helicase

• Polymerase III

• Ligase

You isolate a cell strain in which the joining together of Okazaki fragments is impaired and suspect that a mutation has occurred in an enzyme found at the replication fork. Which enzyme is most likely to be mutated?

• Ligase

• Polymerase I

• Polymerase III

• Topoisomerase

Which is the strand that replicates just as quickly as the replication fork opens up?

• Leading strand

• Lagging strand

• Parent strand

• Daughter strand

Enzyme that catalyse the synthesis of DNA is called

• Polymerase

• Helicase

• Ligase

• Gyrase