DNA Cloning and Recombinant DNA

This are the tools in recombinant DNA technology except

• Modifying enzyme

• DNA donor

• Host cell

• Lac z

Tags

NGSS.HS-LS3-2

Arrange the correct step in gene cloning

• Cut,isolation,insertion,screening

• Isolation,insertion,cut,transformation

• Isolation,cut,insertion,transformation,screening

• Insertion,transformation, isolation

What is restriction enzyme

• Enzyme in plasmid

• Enzyme that recognize and cut at specific base sequence

• Enzyme that join okazaki fragment

• Enzyme that join the plasmid and gene of interest

What is the example of restriction enzyme

• BamHI

• SamH2

• EkaII

• PovIII

Plasmid is ......

• Host cell

• DNA vector

• Bacteria

• Gene of interest

Plasmid is ......

• Host cell

• DNA cloning vector

• Bacteria

• Gene of interest

Tranformation is....

• Insertion gene of interest into plasmid

• Insertion recombinant plasmid into bacteria

• Isolation plasmid from bacteria

• Cut the DNA donor

During screening,what happen to the bacteria that no have plasmid

• Turn to blue colony

• Turn to purple colony

• Died

• Turn to white colony

State the enzyme that produce sticky enzyme

• PovI

• SmaI

• BamHI

• EcoR2

What is plasmid?

• Modifying enzyme

• Host cell

• DNA cloning vector

• DNA donor