Microarray DNA Virtual Activity

What is transcribed from DNA to produce a protein and is a good indication of whether or not a particular gene is expressed?

• mRNA

• cDNA

• tRNA

• trRNA

Tags

NGSS.HS-LS1-1

What happens after the DNA from the sample is applied to the DNA microarray?

• the mRNA falls off the spot

• the DNA comes apart

• the tRNA will join the complementary strand

• the cDNA will hybridize with the single stranded DNA molecules on the microarray

In the microarray, what does the RED color indicate?

• these genes are not expressed or turned off

• these genes are expressed or turned on in cancer

• these genes are expressed in healthy cells

• there are no genes in this cell

What does the GREEN color indicate in DNA microarray?

• these genes are expressed in cancer

• these genes are not expressed in cancer

• these genes are not expressed in healthy cells

• there are no genes in these cells

What does the YELLOW color indicate in DNA microarray?

• this gene is expressed in cancer cells only

• this gene is expressed in healthy cells only

• there are no genes in the cell

• this gene is expressed in both cancer and healthy cells

From microarray data, what can we conclude?

• genes that cause cancer

• which genes may be affected in cancer

• genes that stop cancer

• which cells have genes

Tags

NGSS.HS-LS3-2

What does BLACK indicate in DNA microarray?

• gene expression in cancer cells

• gene expression in healthy and cancer cells

• no gene expression

• gene expression in healthy cells

Which sequence best describes the process for DNA microarray?

• mRNA Isolation; cDNA synthesis; hybridization;scan and analysis

• cDNA synthesis; scan and analysis; mRNA Isolation; hybridization

• hybridization; cDNA synthesis; scan and analysis

• mRNA Isolation; cDNA synthesis; scan and analysis; hybridization

Determine which statement is not true

• Gene A is only expressed in cancerous tissue

• Gene B is expressed in both tissue samples

• Gene C is expressed in both of the tissue samples

• Gene D is only expressed in healthy tissues

What is the complementary cDNA for the following mRNA: AUG CCG UAA

• TAC GGC ATT

• ATC GGC TAA

• UAG GGC AUU

• UAC CCG UAA