PBS 2020 Quiz 3 Unit 1 DNA Analysis

DNA is a long chain made up of smaller subunits called

• nucleotid

• nucleotides

• Double helix

• Restriction enzymes

What is the purpose of PCR?

• To amplify DNA (make lots of copies of DNA)

• Make Double helixs

• The genetic makeup of an individual

• To break apart the fat in the cell membrane

When running a gel, DNA will move to the ____ end because the DNA has a ____ charge

• Double / helix

• mashing / individual

• positive / negative

• cytosine,/ thymine

A nucleotide is made up of a sugar, a phosphate, and a _____

• Restriction enzyme

• positive molecule

• nitrogenous base

• polymerase chain

Where is DNA found?

• with base pairs

• in a Double helix

• the nucleotides

• the nucleus

DNA is and does what?

• strong bonds that hold nucleotides together

• multiple genes put together to form an organism

• ribonucleic acid. Sequence of nucleotides arranged in a specific order, contains instructions for a living organism

• Deoxyribonucleic acid. Sequence of nucleotides arranged in a specific order, contains instructions for a living organism

The pentagon represents...

• Five carbon sugar

• the nucleus

• Adenine and Guanine

• Phosphate

A Gene is...

• Deoxyribonucleic acid

• multiple genes put together to form an organism

• the sequence of nucleotides

• sequence of nucleotides that code for a specific protein

Tags

NGSS.HS-LS1-1

A Genome is....

• Deoxyribonucleic acid

• Purines: Adenine and guanine

  Pyrimidines: Cytosine, Thymine, and Uracil

• a nucleotide

• multiple genes put together to form an organism. These are in the same order for each species of organism

Tags

NGSS.HS-LS3-2

What are the steps of gel electrophoresis?

• 1. Make the gel

  2. Set up the gel apparatus

  3. Load the DNA sample into the gel

  4. Hook up the electrical current and run the gel

  5. Stain the gel and analyze the results

• Purines: Adenine and guanine

  Pyrimidines: Cytosine, Thymine, and Uracil

• A solution of DNA molecules is placed in a gel.

• 1. move extracted DNA into PCR tube

  2. add primer 1 to PCR tube

  3. add primer 2

  4. add nucleotide to the PCR tube

  5. add DNA polymers to the PCR tube

  6. Place DNA PCR tube into DNA thermal cycler