Electrophoresis and PCR

This laboratory procedure is known as

• CLONING

• gel electrophoresis

• chromatography

• use of a dichotomous key

Gel electrophoresis is a laboratory technique designed to separate molecules such as DNA, RNA, or proteins based upon what property?

• size

• Shape

• Color

• number of amino acids

In preparation for an electrophoresis procedure, enzymes are added to DNA in order to

• convert the DNA into gel

• cut the DNA into fragments

• change the color of the DNA

• produce longer sections of DNA

What types of enzymes are used to cut strands of DNA into pieces before placing the DNA in a gel for gel electrophoresis?

• truncation enzymes

• inscription enzymes

• digestive enzymes

• restriction enzymes

How are buffers used in Electrophoresis?

• Conduct electricity

• Remove salts

• Add extension energy

• Dissolve excess DNA

What is the function of the gel in Gel Electrophoresis?

• extract DNA from the liquid

• filter the DNA as it moves through the system

• buffer the DNA so it keeps moving

• charge the DNA so it will move to the opposite side of the system

Provides electrical current to move the DNA down the gel

• Power Source

• Lane

• Well

• Tank

• Marker / DNA Ladder

What similarity between a blood sample collected at the site of a robbery and that of a suspect would indicate that the suspect was most likely responsible?

• if the DNA fingerprint of the suspect matches the DNA fingerprint of the sample collected

• if the blood group of the suspect matches the blood group of the sample collected

• If the suspect's clothing matches the description of the perpetrator

• If the suspect's alibi is inconsistent with the timeline of the robbery

When using gel electrophoresis to analyze samples of DNA, which explanation

best describes why some strands of DNA travel further in the gel than others?

• Strands of DNA are different sizes, and the smaller strands travel further than larger strands.

• Strands of DNA have different charges, and the more positively charged strands travel further than the negatively charged strands.

• Strands of DNA have different shapes, and the more aerodynamic strands travel further than the less aerodynamic strands.

• Strands of DNA have different densities, and the lighter strands travel further than the heavier strands.

What is a practical application for the process illustrated?

• cloning

• DNA fingerprinting

• gene regulation

• protein synthesis

Tags

NGSS.HS-LS3-2