Aseptic Technique

When streaking a plate, the loop should be flamed

• At the beginning and end 
•  At the beginning and end and between the first 2 zones 
• At the beginning and end and between the second and third zone 
• At the beginning and end and between each zone 

What is the first thing you should do during aseptic culture transfer technique?

• put on gloves

• wash your hands

• sterilize your workspace

The purpose of streaking a plate is to obtain 

• large quantities of bacteria
• bacteria in pure culture

When streaking a plate, you should go back over the last area

• never
• 1-2 times
• 3-4 times
• 8-10 times

The bacteriology loop should be sterilized in what part of the Bunsen burner flame? 

• in the bright blue cone
• just above the bright blue cone
• above the flame
• at the base of the flame

What is the last thing you should do?

• take off your gloves
• wash your hands
• sterilize your workspace

For inoculating into a liquid culture, which one of the following is the most appropriate transfer tool

• Swab

• Loop

• Needle

• Glass rod

How can we avoid the agar plate being cross contaminated with unwanted microorganisms when it is open? Pick all that apply?

• Work near a bunsen

• Wipe the surface of the agar with antiseptic

• Minimise the time the lid is open and close it as soon as you are finished inoculation

• Pass the agar through the bunsen flame

What is the goal of aseptic techniques

• To prevent contamination of the worker only

• To prevent contamination of the environment only

• To prevent contamination of the worker and the environment

Before incubation the plate should be...

• Sealed completely with tape

• Sealed with four pieces of tape

• Sealed with two pieces of tape

• Doesn't need sealing