DNA Sequence

What enzyme is used to copy DNA

• Ligase

• Helicase

• Primase

• Polymerase

What do Dideoxy bases do when added to a growing DNA strand?

• Shorten it

• Lengthen it

• Stop it

• Destroy it

When DNA is sorted through electrophoresis, which strands travel furthest?

• Longest

• Shortest

• Most negative

• Most Hydrogen bonds

What is the correct sequence that corresponds with this DNA sequencing chromatogram?

• TCATGGCA

• AGCACCGT

• CACGTTGC

• CTGCAACA

How are regular nucleotides and dideoxynucleotides different? (check all that apply)

• Regular nucleotides are fluorescently labeled, but dideoxy nucleotides are not.

• Dideoxy nucleotides stop the elongation of the DNA strand during PCR.

• Dideoxy nucleotides are for making RNA, but regular nucleotides are for making DNA.

• Dideoxy nucleotides are fluorescently labeled, but regular nucleotides are not.

Gel electrophoresis enables scientists to

• separate DNA fragments.
• combine DNA fragments.
• count the genes in DNA.
• insert DNA in cells.

Dideoxynucleoside triphosphates (ddNTPs) are used in sequencing DNA because:

• ddNTPs are fluorescent.

• ddNTPs are incorporated very efficiently into DNA by DNA polymerase.

• ddNTPs cannot be incorporated into DNA by DNA polymerase.

• ddNTPs prevent further DNA synthesis once they are incorporated into the DNA sequence.

A PCR reaction that continues for 30 cycles will produce approximately how many PCR products from a single template DNA molecule?

• 64

• 128,000

• around a million

• around a billion

DNA has a _________ charge.

• positive

• opposite

• negative

• attractive

Which of the following processes uses PCR, adds dideoxynucleotides, and completes the process with gel electrophoresis?

• DNA profiling

• STR

• VNTR

• DNA sequencing